top of page


51        Interpretation of cell mechanical experiments in microfluidic systems depend on the choice of cellular shape descriptors.

Fregin B, Biedenweg D, Otto O. Biomicrofluidics 2022 Apr 28; 16 (024109). doi: 10.1063/5.0084673

50         Switching in the expression pattern of actin isoforms marks the onset of contractility and distinct mechanodynamic behavior during cardiomyocyte differentiation.
Pires RH, Dau TH, Manu E, Shree N, Otto O. Physiol Rep. 2022 Feb;10(3):e15171. doi: 10.14814/phy2.15171.

49         Ex vivo anticoagulants affect human blood platelet biomechanics with implications for high-throughput functional mechanophenotyping.
Sachs L, Wesche J, Lenkeit L, Greinacher A, Bender M, Otto O, Palankar R.

Commun Biol. 2022 Jan 21;5(1):86. doi: 10.1038/s42003-021-02982-6.

48         Machine learning assisted real-time deformability cytometry of CD34+ cells allows to identify patients with myelodysplastic syndromes.
Herbig M, Jacobi A, Wobus M, Weidner H, Mies A, Kräter M, Otto O, Thiede C, Weickert MT, Götze KS, Rauner M, Hofbauer LC, Bornhäuser M, Guck J, Ader M, Platzbecker U, Balaian E.

Sci Rep. 2022 Jan 18;12(1):870. doi: 10.1038/s41598-022-04939-z.

47         Inhibition of the NLRP3/IL-1β axis protects against sepsis-induced cardiomyopathy.
Busch K, Kny M, Huang N, Klassert TE, Stock M, Hahn A, Graeger S, Todiras M, Schmidt S, Chamling B, Willenbrock M, Groß S, Biedenweg D, Heuser A, Scheidereit C, Butter C, Felix SB, Otto O, Luft FC, Slevogt H, Fielitz J. J Cachexia Sarcopenia Muscle. 2021 Dec;12(6):1653-1668. doi: 10.1002/jcsm.12763.

46         Inactivation of Cerebral Cavernous Malformation Genes Results in Accumulation of von Willebrand Factor and Redistribution of Weibel-Palade Bodies in Endothelial Cells.
Much CD, Sendtner BS, Schwefel K, Freund E, Bekeschus S, Otto O, Pagenstecher A, Felbor U, Rath M, Spiegler S. Front Mol Biosci. 2021 Jul 9;8:622547. doi: 10.3389/fmolb.2021.622547.

45          AQP8 is a crucial H2O2 transporter in insulin-producing RINm5F cells.
Krüger C, Waldeck-Weiermair M, Kaynert J, Pokrant T, Komaragiri Y, Otto O, Michel T, Elsner M.

Redox Biol. 2021 Jul;43:101962. doi: 10.1016/j.redox.2021.101962.

44          Microfluidic Fabrication of Click Chemistry-Mediated Hyaluronic Acid Microgels: A Bottom-Up Material Guide to Tailor a Microgel's Physicochemical and Mechanical Properties.

Heida T, Otto O, Biedenweg D, Hauck N, Thiele J.

Polymers (Basel). 2020 Aug 6;12(8):1760. doi: 10.3390/polym12081760.

43          Label-free on chip quality assessment of cellular blood products using real-time deformability cytometry.

Aurich K, Fregin B, Palankar R, Wesche J, Hartwich O, Biedenweg D, Nguyen TH, Greinacher A, Otto O.

Lab Chip. 2020 Jun 30;20(13):2306-2316. doi: 10.1039/d0lc00258e.

42          High-throughput cell and spheroid mechanics in virtual fluidic channels.

Panhwar MH, Czerwinsiki F, Dabbiru VAS, Komaragiri Y, Fregin B, Biedenweg D, Nestler P, Pires RH and Otto O.

Nat Commun  2020 May 04. doi: 10.1038/s41467-020-15813-9. Online ahead of print.

41          A comparison of microfluidic methods for high-throughput cell deformability measurements.
Urbanska M, Muñoz HE, Shaw Bagnall J, Otto O, Manalis SR, Di Carlo D, Guck J.
Nat Methods. 2020 Apr 27. doi: 10.1038/s41592-020-0818-8. Online ahead of print.

40          Purifying stem cell-derived red blood cells: a high-throughput label-free downstream processing strategy based on microfluidic spiral inertial separation and membrane filtration.
Guzniczak E, Otto O, Whyte G, Chandra T, Robertson NA, Willoughby N, Jimenez M, Bridle H.
Biotechnol Bioeng. 2020 Feb 26. doi: 10.1002/bit.27319. Online ahead of print.

39          Deformability-induced Lift Force in Spiral Microchannels for Cell Separation

Guzniczak E, Otto O, Whyte G, Willoughby N, Jimenez M, Bridle H

Lab Chip. 2020 Jan 9. doi: 10.1039/c9lc01000a.

38          Cardiomyocyte mechanodynamics under conditions of actin remodelling.

Pires RH, Shree N, Manu E, Guzniczak E, Otto O.

Philos Trans R Soc Lond B Biol Sci. 2019 Nov 5. 374 (1786), doi: 10.1098/rstb.2019.0081


37           High-throughput single-cell rheology in complex samples by dynamic real-time deformability cytometry.

Fregin B, Czerwinski F, Biedenweg D, Girardo S, Gross S, Aurich K, Otto O.

Nat Commun. 2019 Jan 24;10(1):415. doi: 10.1038/s41467-019-08370-3.


36           Tafazzin-dependent cardiolipin composition in C6 glioma cells correlates with changes in mitochondrial and cellular functions, and cellular proliferation.

Gürtler S, Wolke C, Otto O, Heise N, Scholz F, Laporte A, Elsner M, Jörns A, Weinert S, Döring M, Jansing S, Gardemann A, Lendeckel U, Schild L.

Biochim Biophys Acta Mol Cell Biol Lipids. 2019 Jan 9;1864(4):452-465. doi: 10.1016/j.bbalip.2019.01.006.


35           Cell number in mesenchymal stem cell aggregates dictates cell stiffness and chondrogenesis.

Sarem M, Otto O, Tanaka S, Shastri VP.

Stem Cell Res Ther. 2019 Jan 10;10(1):10. doi: 10.1186/s13287-018-1103-y.


34           Impact of poloxamer 188 (Pluronic F-68) additive on cell mechanical properties, quantification by real-time deformability cytometry.

Guzniczak E, Jimenez M, Irwin M, Otto O, Willoughby N, and Bridle H.

Biomicrofluidics. 2018 Aug 22; 12(4):044118. doi: 10.1063/1.5040316


33           Biallelic CCM3 mutations cause a clonogenic survival advantage and endothelial cell stiffening.

Schwefel K, Spiegler S, Ameling S, Much CD, Pilz RA, Otto O, Völker U, Felbor U, Rath M.

J Cell Mol Med. 2018 Dec 13. doi: 10.1111/jcmm.14075.


32           No Escape-A Novel Tool to Quantify Platelet Intra-Thrombus Movements.

Bender M, Otto O.

Thromb Haemost. 2018 Sep;118(9):1515-1516. doi: 10.1055/s-0038-1668587.


31           Statistics for real-time deformability cytometry: Clustering, dimensionality reduction, and significance testing.

Herbig M, Mietke A, Müller P, Otto O.

Biomicrofluidics. 2018 Jun 4;12(4):042214. doi: 10.1063/1.5027197.


30           Real-time fluorescence and deformability cytometry.

Rosendahl P, Plak K, Jacobi A, Kraeter M, Toepfner N, Otto O, Herold C, Winzi M, Herbig M, Ge Y, Girardo S, Wagner K, Baum B, Guck J.

Nat Methods. 2018 May;15(5):355-358. doi: 10.1038/nmeth.4639.


29           Detection of human disease conditions by single-cell morpho-rheological phenotyping of blood.

Toepfner N, Herold C, Otto O, Rosendahl P, Jacobi A, Kräter M, Stächele J, Menschner L, Herbig M, Ciuffreda L, Ranford-Cartwright L, Grzybek M, Coskun Ü, Reithuber E, Garriss G, Mellroth P, Henriques-Normark B, Tregay N, Suttorp M, Bornhäuser M, Chilvers ER, Berner R, Guck J.

Elife. 2018 Jan 13;7. pii: e29213. doi: 10.7554/eLife.29213.


28           Real-Time Deformability Cytometry: Label-Free Functional Characterization of Cells.

Herbig M, Kräter M, Plak K, Müller P, Guck J, Otto O.

Methods Mol Biol. 2018;1678:347-369. doi: 10.1007/978-1-4939-7346-0_15.


27           Toxicity and Immunogenicity in Murine Melanoma following Exposure to Physical Plasma-Derived Oxidants.

Bekeschus S, Rödder K, Fregin B, Otto O, Lippert M, Weltmann KD, Wende K, Schmidt A, Gandhirajan RK.

Oxid Med Cell Longev. 2017;2017:4396467. doi: 10.1155/2017/4396467.


26           Bone marrow niche-mimetics modulate HSPC function via integrin signaling.

Kräter M, Jacobi A, Otto O, Tietze S, Müller K, Poitz DM, Palm S, Zinna VM, Biehain U, Wobus M, Chavakis T, Werner C, Guck J, Bornhauser M.

Sci Rep. 2017 May 31;7(1):2549. doi: 10.1038/s41598-017-02352-5.


25           Actin stress fiber organization promotes cell stiffening and proliferation of pre-invasive breast cancer cells.

Tavares S, Vieira AF, Taubenberger AV, Araújo M, Martins NP, Brás-Pereira C, Polónia A, Herbig M, Barreto C, Otto O, Cardoso J, Pereira-Leal JB, Guck J, Paredes J, Janody F.

Nat Commun. 2017 May 16;8:15237. doi: 10.1038/ncomms15237.


24           High-throughput cell mechanical phenotyping for label-free titration assays of cytoskeletal modifications.

Golfier S, Rosendahl P, Mietke A, Herbig M, Guck J, Otto O.

Cytoskeleton (Hoboken). 2017 Aug;74(8):283-296. doi: 10.1002/cm.21369.


23           Plasmodium falciparum erythrocyte-binding antigen 175 triggers a biophysical change in the red blood cell that facilitates invasion.

Koch M, Wright KE, Otto O, Herbig M, Salinas ND, Tolia NH, Satchwell TJ, Guck J, Brooks NJ, Baum J.

Proc Natl Acad Sci U S A. 2017 Apr 18;114(16):4225-4230. doi: 10.1073/pnas.1620843114.


22           Enlightening discriminative network functional modules behind Principal Component Analysis separation in differential-omic science studies.

Ciucci S, Ge Y, Durán C, Palladini A, Jiménez-Jiménez V, Martínez-Sánchez LM, Wang Y, Sales S, Shevchenko A, Poser SW, Herbig M, Otto O, Androutsellis-Theotokis A, Guck J, Gerl MJ, Cannistraci CV.

Sci Rep. 2017 Mar 13;7:43946. doi: 10.1038/srep43946.


21           The F-actin modifier villin regulates insulin granule dynamics and exocytosis downstream of islet cell autoantigen 512.

Mziaut H, Mulligan B, Hoboth P, Otto O, Ivanova A, Herbig M, Schumann D, Hildebrandt T, Dehghany J, Sönmez A, Münster C, Meyer-Hermann M, Guck J, Kalaidzidis Y, Solimena M.

Mol Metab. 2016 Jun 10;5(8):656-668. doi: 10.1016/j.molmet.2016.05.015.


20           A pH-driven transition of the cytoplasm from a fluid- to a solid-like state promotes entry into dormancy.

Munder MC, Midtvedt D, Franzmann T, Nüske E, Otto O, Herbig M, Ulbricht E, Müller P, Taubenberger A, Maharana S, Malinovska L, Richter D, Guck J, Zaburdaev V, Alberti S.

Elife. 2016 Mar 22;5. pii: e09347. doi: 10.7554/eLife.09347.


19           Mechanical phenotyping of primary human skeletal stem cells in heterogeneous populations by real-time deformability cytometry.

Xavier M, Rosendahl P, Herbig M, Kräter M, Spencer D, Bornhäuser M, Oreffo RO, Morgan H, Guck J, Otto O.

Integr Biol (Camb). 2016 May 16;8(5):616-23. doi: 10.1039/c5ib00304k.


18           Real-time deformability cytometry as a label-free indicator of cell function.

Otto O, Rosendahl P, Golfier S, Mietke A, Herbig M, Jacobi A, Topfner N, Herold C, Klaue D, Girardo S, Winzi M, Fischer-Friedrich E, Guck J.

Conf Proc IEEE Eng Med Biol Soc. 2015;2015:1861-4. doi: 10.1109/EMBC.2015.7318744.


17           Extracting Cell Stiffness from Real-Time Deformability Cytometry: Theory and Experiment.

Mietke A, Otto O, Girardo S, Rosendahl P, Taubenberger A, Golfier S, Ulbricht E, Aland S, Guck J,
Fischer-Friedrich E.

Biophys J. 2015 Nov 17;109(10):2023-36. doi: 10.1016/j.bpj.2015.09.006.


16           Association of the EGF-TM7 receptor CD97 expression with FLT3-ITD in acute myeloid leukemia.

Wobus M, Bornhäuser M, Jacobi A, Kräter M, Otto O, Ortlepp C, Guck J, Ehninger G, Thiede C, Oelschlägel U.

Oncotarget. 2015 Nov 17;6(36):38804-15. doi: 10.18632/oncotarget.5661.


15           Myosin II Activity Softens Cells in Suspension.

Chan CJ, Ekpenyong AE, Golfier S, Li W, Chalut KJ, Otto O, Elgeti J, Guck J, Lautenschläger F.

Biophys J. 2015 Apr 21;108(8):1856-69. doi: 10.1016/j.bpj.2015.03.009.


14           Real-time deformability cytometry: on-the-fly cell mechanical phenotyping.

Otto O, Rosendahl P, Mietke A, Golfier S, Herold C, Klaue D, Girardo S, Pagliara S, Ekpenyong A, Jacobi A, Wobus M, Töpfner N, Keyser UF, Mansfeld J, Fischer-Friedrich E, Guck J.

Nat Methods. 2015 Mar;12(3):199-202, 4 p following 202. doi: 10.1038/nmeth.3281.


13           Camera-based three-dimensional real-time particle tracking at kHz rates and Ångström accuracy.

Huhle A, Klaue D, Brutzer H, Daldrop P, Joo S, Otto O, Keyser UF, Seidel R.

Nat Commun. 2015 Jan 7;6:5885. doi: 10.1038/ncomms6885.


12           Dynamic operation of optical fibres beyond the single-mode regime facilitates the orientation of biological cells.

Kreysing M, Ott D, Schmidberger MJ, Otto O, Schürmann M, Martín-Badosa E, Whyte G, Guck J.

Nat Commun. 2014 Nov 20;5:5481. doi: 10.1038/ncomms6481.


11           Rapid internal contraction boosts DNA friction.

Otto O, Sturm S, Laohakunakorn N, Keyser UF, Kroy K.

Nat Commun. 2013;4:1780. doi: 10.1038/ncomms2790.


10           DNA interactions in crowded nanopores.

Laohakunakorn N, Ghosal S, Otto O, Misiunas K, Keyser UF.

Nano Lett. 2013 Jun 12;13(6):2798-802. doi: 10.1021/nl401050m.


9              Voltage-driven transport of ions and DNA through nanocapillaries.

Steinbock LJ, Lucas A, Otto O, Keyser UF.

Electrophoresis. 2012 Dec;33(23):3480-7. doi: 10.1002/elps.201100663.


8              Note: Direct force and ionic-current measurements on DNA in a nanocapillary.

Otto O, Steinbock LJ, Wong DW, Gornall JL, Keyser UF.

Rev Sci Instrum. 2011 Aug;82(8):086102. doi: 10.1063/1.3623502.


7              Simple reconstitution of protein pores in nano lipid bilayers.

Gornall JL, Mahendran KR, Pambos OJ, Steinbock LJ, Otto O, Chimerel C, Winterhalter M, Keyser UF.

Nano Lett. 2011 Aug 10;11(8):3334-40. doi: 10.1021/nl201707d.


6              Micro-rheology on (polymer-grafted) colloids using optical tweezers.

Gutsche C, Elmahdy MM, Kegler K, Semenov I, Stangner T, Otto O, Ueberschär O, Keyser UF, Krueger M, Rauscher M, Weeber R, Harting J, Kim YW, Lobaskin V, Netz RR, Kremer F.

J Phys Condens Matter. 2011 May 11;23(18):184114. doi: 10.1088/0953-8984/23/18/184114.


5              Probing DNA with micro- and nanocapillaries and optical tweezers.

Steinbock LJ, Otto O, Skarstam DR, Jahn S, Chimerel C, Gornall JL, Keyser UF.

J Phys Condens Matter. 2010 Nov 17;22(45):454113. doi: 10.1088/0953-8984/22/45/454113.


4              Real-time particle tracking at 10,000 fps using optical fiber illumination.

Otto O, Czerwinski F, Gornall JL, Stober G, Oddershede LB, Seidel R, Keyser UF.

Opt Express. 2010 Oct 25;18(22):22722-33. doi: 10.1364/OE.18.022722.


3              Detecting DNA folding with nanocapillaries.

Steinbock LJ, Otto O, Chimerel C, Gornall J, Keyser UF.

Nano Lett. 2010 Jul 14;10(7):2493-7. doi: 10.1021/nl100997s. Erratum in: Nano Lett. 2013 Jul 10;13(7):3444.


2              Single colloid electrophoresis.

Semenov I, Otto O, Stober G, Papadopoulos P, Keyser UF, Kremer F.

J Colloid Interface Sci. 2009 Sep 1;337(1):260-4. doi: 10.1016/j.jcis.2009.05.017.


1              Optical tweezers with 2.5 kHz bandwidth video detection for single-colloid electrophoresis.

Otto O, Gutsche C, Kremer F, Keyser UF.

Rev Sci Instrum. 2008 Feb;79(2 Pt 1):023710. doi: 10.1063/1.2884147.

bottom of page